Detection and Frequency of Enterotoxin (cpa,cpe) Genes of Clostridium perfringens Isolated from Dehydrated Vegetables by PCR
نویسندگان
چکیده
Detection and Frequency of Enterotoxin (cpa,cpe) Genes Clostridium perfringens Isolated from Dehydrated Vegetables by PCR
منابع مشابه
Detection, cloning, and sequencing of the enterotoxin gene of Clostridium perfringens type C isolated from goat
Clostridium perfringens enterotoxin (CPE) is an important virulence factor involved in C. perfringens food poisoning and other gastrointestinal illnesses. In our experiment a Clostridium perfringens enterotoxin gene (cpe) positive type C strain was obtained by multiplex PCR from 34 C. perfringens isolates from goat in Guizhou Province, China. Th en a pair of specifi c primers was designed and s...
متن کاملPRODUCTION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES TO CLOSTRIDIUM PERFRINGENS ENTEROTOXIN DERIVED FROM DIFFERENT ANIMAL SPECIES
The enterotoxins (ETs) of Clostridium perfringens isolated from enterotoxemic or diarrheic alpacas, pigs, calves, dogs, and horses were obtained from sporulated cell extracts. The ETs from alpaca, pig, and calf isolates were chromatographed on Sephadex G-lOO. Monoclonal antibodies (MAbs) against ETs derived from alpaca, pig, and calf C. perfringens isolates were produced. The MAbs were used...
متن کاملIdentification and Characterization of a New Enterotoxin Produced by Clostridium perfringens Isolated from Food Poisoning Outbreaks
There is a strain of Clostridium perfringens, W5052, which does not produce a known enterotoxin. We herein report that the strain W5052 expressed a homologue of the iota-like toxin components sa and sb of C. spiroforme, named Clostridium perfringens iota-like enterotoxin, CPILE-a and CPILE-b, respectively, based on the results of a genome sequencing analysis and a systematic protein screening. ...
متن کاملDevelopment of real-time PCR for the detection of Clostridium perfringens in meats and vegetables.
A real-time PCR assay was developed and validated inhouse specifically for the detection of Clostridium perfringens (Cl. perfringens) in meats and vegetables by comparing with the culture method. The detection limit of the real-time PCR assay in phosphate-buffered saline was 10² CFU/ml. When the two methods were compared in food samples inoculated with Cl. perfringens, the culture method detect...
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ژورنال
عنوان ژورنال: Journal of Advances in Medical and Biomedical Research
سال: 2021
ISSN: ['2676-6264']
DOI: https://doi.org/10.30699/jambs.29.133.63